Observation of the compatibility of stimulated Raman histology with pathology workflow and genome sequencing

We show that human samples imaged with stimulated Raman histology (SRH) can be successfully used in a typical pathology workflow including hematoxylin and eosin staining (HE), immunohistochemistry, genome sequencing. provide morphological comparison of SRH images the gold standard HE HES (HE saffron) over three normal tissues from various organs (liver, kidney, ileum) two tumoral brain (metastatic adenocarcinoma glioblastoma). Most importantly, we sample has comparable microscopic appearance as control (that was not by SRH) protein expression evaluated immunohistochemistry well similar genetic alterations This suggests direct diagnosis without material preparation nor consumption.